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Microbiology

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Faculty

Dr Poonam Sood Loomba Dir. Professor and Head
Dr Manisha Jain Professor
Dr Abha Sharma Associate Professor
Dr Shivani Tyagi Assistant Professor
Dr Sulmaz Reshi Assistant Professor

Location  of the Department

  • 3rd floor, Academic block, Room no . 301 to 318, GB pant hospital
  • 1st floor, D-block, Room No. 119, Room No 125  and Room no.127, GB pant hospital

Departmental profile:

The department of Microbiology is involved in various investigative, teaching and research activities. Apart from routine isolation/identification and antibiogram of various microbial agents from clinical specimens: isolation and identification of anaerobic bacteria and other specialized investigations are also being carried out. Immunological tests, ameobiasis, hydatid disease and HIV I and II ANA dengue IgM serology, chikunguniya, serology NSI, for dengue antigen are also being carried out inaddition to routine serological/immunological tests. Identification and culture of fungus from various clinical sample i.e. in CSF, Sputum, Pus and Blood of cases of chronic meningitis, endocarditis, lung abscess and brain abscess are also being carried out. In 1984, Hospital Infection control committee was established. Hospital infection control committee is actively involved in monitoring hospital infection and formulating measures to reduce it. Recently, it is actively involved in the Biomedical Waste ManagementProgramme.

In 2015, Mycobacterium detection and sensitivity system (MGIT) was added to the department and Mycobacteriology laboratory was set up. In 2015 automated blood culture system was also installed in the department for fast processing of blood culture samples so as to reduce the TAT (Turn Around Time) for diagnosis of bloodstream infections. In 2019, two Post Graduate seats in MD Microbiology were approved and sanctioned to the department by the Medical Council of India. In 2020, automated RT PCR(TRUENAT) testing for SARS - COV 2 has been started in the department. Soon a PCR laboratory will be developed in the department for molecular diagnosis of infectious diseases

List of services:
The microbiology Department provides diagnostic services to the clinical departments.

Diagnostic bacteriology:

  • Gram staining and culture and antibiotic sensitivity of bacteria from clinical samples.
  • Gram staining and culture and antibiotic sensitivity for anaerobic bacteria from clinical samples.
  • Special staining:- ZN Stain, Giemsa staining and Mucicarmine stain
  • Culture& sensitivity test for M tuberculosis

Diagnostic parasitology

  • Stool direct microscopic examination for parasitic ova/eyst
  • Modified acid fast staining for opportunistic parasites 

Serology

  • Malarial antigen, widal, typhi dot, ASO/CRP, Dengue IgM, cryptococcal antigen and VDRL, rapid card tests for HIV, HBV,HCV
  • Weil Felix reaction and ELISA for scrub typhus
  • ELISA for measles antibody
  • ANA by LIA (line immunoassay)
  • ANA by immunofluorescence assay
  • VIDAS: EBV/NSI / Clostridiodes difficile

Diagnostic mycology-Direct KOH mount, calcoflor staining, fungal culture and identification, Sensitivity of yeast, Cryptococcal latex agglutination test

Molecular Laboratory:

  • TRUENAT tasting- for SARS-COV 2/TB/HBV/HCV/ Clostridiodes difficile
  • RT-PCR for SARS-COV 2
  • Multiplex PCR for meningoencephalitis panel (Biofire)
  • Multiplex PCR for HBV,HCV

Hospital Infection control

  • Air bacteriology
  • Water Bacteriology
  • Nasal Swab
  • Biological indicator testing
  • Swabs from equipment
  • Disinfectant testing.
  • Sterility test :- I.V. Fluid Surgical drapes/gowns

Mycobacteriology:

  • TB Culture and sensitivity (MGIT)

Biomedical waste management 

Time and location of receiving samples:
Dept. Of Microbiology, 3 rd floor, Academic Block, Room No. 301 to 318, G.B. Pant Hospital.

Day & Time OPD Central Collection Centre Routine Samples from Wards
  Serology Culture Serology Culture
Monday to Friday
8 am 1 pm		 D-125 A-318 D-119 A-308
Saturday
8 am 11 am		 D-125 A-318 D-119 A-308

Clinical Samples for RT-PCR for SARS-COR-2: D-127

Emergency samples for Culture after 4 p.m : D-127

  • CSF, blood, brain abscess, ascitic fluid, pleural fluid, pericardial fluid, BAL

Emergency samples for Serology: D-127

  • Rapid card test for anti HIV, HBsAg, and anti HCV

Documents required for the service:

  • Patient information, relevant clinical details and history of antibiotic therapy.

Required fee, if any:

  • These tests are free of cost for inpatients and outdoor patients of G.B. Pant Hospital and other Govt. hospitals.

Academic activities:

  • Two batches of MD Microbiology post graduate students have passed the final examination
  • Two post graduate (MD Microbiology) students join the department every year.
  • The department actively participates in national/international/state level conferences by presenting scientific papers and delivering CME lectures.
  • Research papers are published by the department in indexed journals

Future plans:

  • To get the laboratory accredited by NABL.
  • To set up an advanced virology research center.
  • To set up advanced Mycobacteriology lab for anti tubercular drug susceptibility.
  • To set up advanced molecular laboratory for genomic sequencing and surveillance.
  • To increase the MD seats from to four per year.
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